CONSULTATION

Free consultation is available to discuss potential projects and/or collaborations (contact us).

WHAT ARE YOU AIMING FOR?

Knock-out (KO)

  • Single gene

  • Several genes (multiplex)

Cas9 + gRNA(s)

Knock-in (KI)

  • ​Tag

      N-terminal tag

      C-terminal tag

      internal tag

  • Point mutation

  • Transgene (BAC, cDNA)

Cas9 + gRNA

        + Donor

conditional Knock-out (cKO)

  • Site-specific recombinases

      Cre / loxP

      Dre / rox

      Flpo / FRT

      Vika / vox

  • Tamoxifen inducible (ERT2)

  • Auxin-inducible degron system

Cas9 + gRNA

        + Donor

DELIVERY OF COMPONENTS

DELIVERY OF COMPONENTS

Depending on your organism/cell line of choice Cas9 and gRNA can be delivered as Plasmid, RNA or Protein. We will advice you on the best delivery option and generate the reagents. In case you require a donor template we will design and generate it for you.

ETHICAL STATEMENT

In accordance with the 3Rs please check whether targeted ES cells or mice exist for your gene of interest.

Look here: findmice.org