CRISPR CLUB

We run a quarterly CRISPR club to bring together researchers with a working interest in CRISPR/Cas9 aiming to facilitate further inter-departmental communication and collaboration within KCL. The CRISPR club takes place at New Hunt's House, 4th floor -  large seminar room.

Next CRISPR club - TBC

• Previous CRISPR clubs

  12/2019 |  “CRISPR correction of ALS-linked TDP-43 mutations in patient hiPSCs”

                   Peter Harley - Centre for Stem Cells & Regenerative Medicine

                   "PrimeEditingOliver Baker

  10/2019 |  "Different Functions and Mechanisms of LARP4 RNA-Binding Proteins in Prostate Cancer" 

                   Jen Coleman - Centre for Craniofacial and Regenerative Biology

                   "Different genome editing strategies to assess the role of hippocampus neurogenesis in Depression and            

                    Alzheimer's Disease" Thomas Berger - IoPPP

  03/2019 |  “Attempting to use Crispr-Cas9 to edit sensory neuron genes in vivo” 

                   Choi Dongchan - King's College London

  01/2019 |  "Modelling intrahepatic cholestasis secondary to TJP2 gene mutation using hiPSC and 3D microwell"

                  Chaozheng Li (Rex) -  King's Centre for Stem Cells & Regenerative Medicine

                  "From concept to clinic; MaxCyte cell engineering platforms for CRISPR based therapies"

                  Payal Roychoudhury, PhD MBA – Field Applications Scientist, MaxCyte UK

  10/2018 |  "CRISPRa reprogramming to pluripotency” Ras Trokovic, Core Manager, Biomedicum Stem Cell Center,                                         GoEditStem platform, HILIFE University of Helsinki

                   "Improving CRISPR editing efficiencies in challenging primary and stem cells" Kevin Holden, Head of                                           Synthetic Biology (Synthego Corporation)

   09/2018 | "Design strategies for knock-ins (incl. point mutations)" - Oliver Baker

   08/2018 | "CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells” 

                    Marc-David Ruepp

   06/2018 | "gRNA design using online tools" - Oliver Baker

   05/2018 | "How to design a good gRNA?" - Oliver Baker

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PUBLICATIONS

Andrea J. Korecki, Jack W. Hickmott, Siu Ling Lam, Lisa Dreolini, Anthony Mathelier, Oliver Baker, Claudia Kuehne, Russell J. Bonaguro, Jillian Smith, Chin-Vern Tan, Michelle Zhou, Daniel Goldowitz, Jan M. Deussing, A. Francis Stewart, Wyeth W. Wasserman, Robert A. Holtand, Elizabeth M. Simpson

Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele, Genetics (2019)

PDF version here

Baker O*, Karimova. M*, Camgoz A, Naumann R, Buchholz F, Anastassiadis K

A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination, Scientific reports (2018)

PDF version here

Baker O, Tsurkan S, Fu J, Klink B, Rump A, Obst M, Kranz A, Schröck E, Anastassiadis K, Stewart AF.

The contribution of homology arms to nuclease-assisted genome engineering, Nucleic Acids Research (2017)

PDF version here

Baker O, Gupta A, Obst M, Zhang Y, Anastassiadis K, Fu J, Stewart AF.

RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses, Scientific reports (2016)

PDF version here

Ferdinando Scavizzi, Edward Ryder, Stuart Newman et al.

Blastocyst genotyping for quality control of mouse mutant archives: an ethical and economical approach, Transgenic Res (2015)

PDF version here

Edward Ryder, Brendan Doe, Diane Gleeson, Richard Houghton, Priya Dalvi, Evelyn Grau, Bishoy Habib, Evelina Miklejewska, Stuart Newman et al.,

Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase, Transgenic Res (2014)

PDF version here

TEACHING

•  ‘CRISPR Design & Strategy’ (2-day workshop for PhD students)

   11/2019 | King's College London, London

•  ‘Exploiting CRISPR for gene tagging and large KI’ (invited speaker)

   'CRISPR design workshop'

   05/2019 | University of Liverpool, Liverpool

•  ‘Genetic Engineering of Mammalian Stem Cells’ (invited Guest speaker)

   03/2019 | Wellcome Trust Sanger Institute, Cambridge

•  ‘CRISPR Design & Strategy’ (2-day workshop for internal PhD students)

   12/2018 | King's College London, London

•  ‘CRISPR Mini Masterclass’ (1-day workshop for internal PhD students)

   05/2018 | King's College London, London

•  ‘Genetic Engineering of Mammalian Stem Cells’ (invited Guest speaker)

   03/2018 | Wellcome Trust Sanger Institute, Cambridge

•  iGEM seminar (invited talk)

   02/2018 | King's College London, London

•  MSci module: Integrative Pharmacology and Physiology for Research (seminar)

    'Strategies for the generation of genetically altered (GA) animals'    

    11/2019 | King's College London, London   

    11/2018 | King's College London, London

    11/2017 | King's College London, London

• 2-day course instructor ‘CRISPR Design & Strategy’

   08/2019 | CamBioScience, Cambridge   

   12/2018 | CamBioScience, Seoul

   12/2018 | CamBioScience, Beijing

   08/2018 | CamBioScience, Cambridge

   06/2018 | CamBioScience, Cambridge

   11/2017 | CamBioScience, Cambridge

   08/2017 | CamBioScience, Seoul   

   06/2017 | CamBioScience, Cambridge   

   03/2017 | CamBioScience, Cambridge

   11/2016 | CamBioScience, Cambridge

    www.cambioscience.com

• 2-week course instructor ‘Genetic Engineering of Mammalian Stem Cells’

   2012 & 2015 | Wellcome Trust Sanger Institute, Cambridge

• 1-week course instructor ‘Recombineering’

   2011 & 2013 | BioInnovation Center, Dresden

CONFERENCES

• Next-generation CRISPR technologies 

   11/2019 | Crick Institute 

• 4th Annual Genome Editing Congress

   11/2019 | London

• Advances in Immuno-Oncology, London (05/2019)

• International Society for Transgenic Technologies meeting, Japan (04/2019)

  'A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination' (selected flash talk and poster)

• Genome Engineering – Research & Applications, Florence (2018)

   Baker et al., ‘The contribution of homology arms to nuclease-assisted genome engineering' (Poster)

• CRISPR core partnership meeting (Merck)

   07/2018 | Barcelona

• 7th Annual Technicians Symposium, Crick Institute, London (02/2018)

    'A crisp intro to CRISPR for custom mouse model generation' (invited talk)

• 3rd Annual Genome Editing Congress

   11/2017 | London

• EMBL-EBI Industry programme: Genome Editing in Drug Discovery (invited talk)

   25-26/10 | Sanger Institute, Hinxton

• ISTT meeting 

   10/2017 | Utah, USA​

• 9th Workshop on Innovative Mouse Models, Leiden (2017)

   Baker et al., 'Targeted gene replacement of 42kb using asymmetric donors in mES cells‘ (selected talk)

• Genome Engineering – Research & Applications, Lisbon (2016)

• CRISPR/Cas9 Symposium, Dresden (2015)

   Baker et al., ‘CRISPR/Cas9 in mice and mammalian cells’ (invited talk)

• 8th Workshop on Innovative Mouse Models, Leiden (2015)

• 7th Workshop on Innovative Mouse Models, Leiden (2014)

   Baker et al., ‘Vika/vox, a novel recombinase system for mammalian systems‘ (selected talk)

• Genome Engineering – Research & Applications, Lucca (2012)

   Baker et al., ‘A convertable Cre system for conditional mutagenesis‘ (Poster)

• Gene Targeting Conference, Vienna (2011)